The journey of a Zebrafish

July 19, 2016

I still remember the day I brought them back from the supplier- Danio, Danio rerio long fins. pH check, temperature check, conductivity stable. Tecniplast was going to be my new favorite laboratory helper and so far we are getting on just great. The fish were maintained at 25 °C in the tanks for a month before we even separated males and females, then for an additional month they were fed on a nutrient rich diet while we gradually increased the temperature (by the touch of a button I may add) to 28 °C.

A Tecniplast 1.7 L slope tank was used for breeding, the tank has a grid which has a gravel textured bottom as well as a slope with less water on one end to activate fish for spawning. Fish weren't fed on the morning prior to breeding to avoid any waste contamination with the embryos. The breeding tank was placed into a buffer water tank to establish and maintain the temperature in the breeding tank between 26 -28°C. Three gravid females and two sexually mature males were removed from the housing tank placed into the 1.7 L breeding tank and left there overnight. One hour after dawn the adult fish were removed by removing the top section of the tank and leaving the eggs housed at the bottom. The eggs were transferred to a hatching tank where the temperature was maintained between 26 -27°C. And the next morning translucent little eggs (not the unfertilized white ones) were everywhere. Ten days later we had around one hundred and fifty free swimming larvae which were fed on Tetramin baby until being fed on crushed adult flakes. It's been two months now...

 And just look at that. In two months (and even less while we optimize the tanks) we will be able to start this all again with "I still remember the day these little guys hatched". It all would have gone full cycle, as the journey of a Zebrafish, helps us setup guidelines for new potential (nano)risks.

 

 

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